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pcfj1662 ph  (Addgene inc)


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    Structured Review

    Addgene inc pcfj1662 ph
    Pcfj1662 Ph, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcfj1662+ph/pMA122+-+peel-1+negative+selection+(Plasmid+%2334873)/pm37313686-223-3-8
    Average 93 stars, based on 41 article reviews
    pcfj1662 ph - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.
    Article Snippet: In brief, the PCR-amplified fragment of pie-1p::gfp::PH::pie-1 3′ UTR was inserted into pCFJ1662 (Addgene #51482, deposited by Erik Jorgensen). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1; Addgene #34873, deposited by Erik Jorgensen) and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with an SL2 trans-splicing sequence and inserting this into pCFJ910 (Addgene #44481, deposited by Erik Jorgensen,) with the pie-1p and pie-1 3′ UTR.

    Article Title: Germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans
    Article Snippet: In brief, the PCR-amplified fragment of pie-1 p:: gfp ::PH:: pie-1 3’UTR was inserted into pCFJ1662 (a gift from Erik Jorgensen, Addgene #51482). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1 , Addgene #34873), and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express the mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with a SL2 trans-splicing sequence and inserted into pCFJ910 (a gift from Erik Jorgensen, Addgene #44481) with the pie-1p and pie-1 3’UTR.

    Injection:

    Article Title: A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.
    Article Snippet: In brief, the PCR-amplified fragment of pie-1p::gfp::PH::pie-1 3′ UTR was inserted into pCFJ1662 (Addgene #51482, deposited by Erik Jorgensen). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1; Addgene #34873, deposited by Erik Jorgensen) and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with an SL2 trans-splicing sequence and inserting this into pCFJ910 (Addgene #44481, deposited by Erik Jorgensen,) with the pie-1p and pie-1 3′ UTR.

    Article Title: Germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans
    Article Snippet: In brief, the PCR-amplified fragment of pie-1 p:: gfp ::PH:: pie-1 3’UTR was inserted into pCFJ1662 (a gift from Erik Jorgensen, Addgene #51482). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1 , Addgene #34873), and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express the mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with a SL2 trans-splicing sequence and inserted into pCFJ910 (a gift from Erik Jorgensen, Addgene #44481) with the pie-1p and pie-1 3’UTR.

    Transgenic Assay:

    Article Title: A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.
    Article Snippet: In brief, the PCR-amplified fragment of pie-1p::gfp::PH::pie-1 3′ UTR was inserted into pCFJ1662 (Addgene #51482, deposited by Erik Jorgensen). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1; Addgene #34873, deposited by Erik Jorgensen) and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with an SL2 trans-splicing sequence and inserting this into pCFJ910 (Addgene #44481, deposited by Erik Jorgensen,) with the pie-1p and pie-1 3′ UTR.

    Article Title: Germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans
    Article Snippet: In brief, the PCR-amplified fragment of pie-1 p:: gfp ::PH:: pie-1 3’UTR was inserted into pCFJ1662 (a gift from Erik Jorgensen, Addgene #51482). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1 , Addgene #34873), and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express the mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with a SL2 trans-splicing sequence and inserted into pCFJ910 (a gift from Erik Jorgensen, Addgene #44481) with the pie-1p and pie-1 3’UTR.

    Selection:

    Article Title: A germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans.
    Article Snippet: In brief, the PCR-amplified fragment of pie-1p::gfp::PH::pie-1 3′ UTR was inserted into pCFJ1662 (Addgene #51482, deposited by Erik Jorgensen). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1; Addgene #34873, deposited by Erik Jorgensen) and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with an SL2 trans-splicing sequence and inserting this into pCFJ910 (Addgene #44481, deposited by Erik Jorgensen,) with the pie-1p and pie-1 3′ UTR.

    Article Title: Germline-specific role for unconventional components of the γ-tubulin complex in Caenorhabditis elegans
    Article Snippet: In brief, the PCR-amplified fragment of pie-1 p:: gfp ::PH:: pie-1 3’UTR was inserted into pCFJ1662 (a gift from Erik Jorgensen, Addgene #51482). .. Plasmid mixtures containing pCFJ1662_PH, pCFJ601, pMA122 ( peel-1 , Addgene #34873), and co-injection markers were injected into N2 young adult worms, and transgenic strains were obtained by hygromycin selection. .. To co-express the mCherry::TBG-1 (genomic DNA) and mCherry::histone H2B, plasmid pNH33 was generated by connecting two genes with a SL2 trans-splicing sequence and inserted into pCFJ910 (a gift from Erik Jorgensen, Addgene #44481) with the pie-1p and pie-1 3’UTR.



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    93
    Addgene inc pcfj1662 ph
    Pcfj1662 Ph, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcfj1662+ph/pMA122+-+peel-1+negative+selection+(Plasmid+%2334873)/pm37313686-223-3-8
    Average 93 stars, based on 1 article reviews
    pcfj1662 ph - by Bioz Stars, 2026-10
    93/100 stars
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